Molecular weight (DA)
190kD
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human GRF-1 (phospho Tyr1087)
Specificity
Phospho-GRF-1 (Y1087) Polyclonal Antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Y1087.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008],
Function
function:Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Tyrosine phosphorylated.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 4 FF domains.,subunit:Interacts with p120GAP.,
Protein name
Rho GTPase-activating protein 35
Other name
ARHGAP35; GRF1; GRLF1; KIAA1722; Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A
Fields
>>Focal adhesion;>>Platelet activation;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton
Human protein sequence Database
Q9NRY4
Mouse protein sequence database
Q91YM2
Rat protein sequence database
P81128
Cellular localization
Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm . Nucleus . Cell membrane . In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin. .
Tissue expression
Detected in neutrophils (at protein level).
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