Molecular weight (DA)
98kD
Immunogen
The antiserum was produced against synthesized peptide derived from human VAV1 around the phosphorylation site of Tyr174. AA range:141-190
Specificity
Phospho-Vav1 (Y174) Polyclonal Antibody detects endogenous levels of Vav1 protein only when phosphorylated at Y174.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012],
Function
domain:The DH domain is involved in interaction with CCPG1.,function:Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.,miscellaneous:'Vav' stands for the sixth letter of the Hebrew alphabet.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:May interact with CCPG1 (By similarity). Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylati
Protein name
Proto-oncogene vav
Other name
VAV1; VAV; Proto-oncogene vav
Fields
>>Rap1 signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>Focal adhesion;>>Natural killer cell mediated cytotoxicity;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Yersinia infection;>>Proteoglycans in cancer;>>Lipid and atherosclerosis
Human protein sequence Database
P15498
Mouse protein sequence database
P27870
Rat protein sequence database
P54100
Cellular localization
intracellular,cytosol,plasma membrane,cell-cell junction,
Tissue expression
Widely expressed in hematopoietic cells but not in other cell types.
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