Molecular weight (DA)
33kD
Immunogen
The antiserum was produced against synthesized peptide derived from human RAD. AA range:41-90
Specificity
Rad GTPase Polyclonal Antibody detects endogenous levels of Rad GTPase protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
similarity:Belongs to the small GTPase superfamily. RGK family.,subunit:Interacts with calmodulin preferentially to the inactive, GDP-form. Binds CAMKII which is capable of phosphorylating RAD in vitro.,tissue specificity:Skeletal and cardiac muscle, and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.,
Function
similarity:Belongs to the small GTPase superfamily. RGK family.,subunit:Interacts with calmodulin preferentially to the inactive, GDP-form. Binds CAMKII which is capable of phosphorylating RAD in vitro.,tissue specificity:Skeletal and cardiac muscle, and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.,
Protein name
GTP-binding protein RAD
Other name
RRAD; RAD; GTP-binding protein RAD; RAD1; Ras associated with diabetes
Human protein sequence Database
P55042
Mouse protein sequence database
O88667
Rat protein sequence database
Cellular localization
Cell membrane .
Tissue expression
Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.
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