Molecular weight (DA)
18kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Myosin regulatory light chain 2 around the phosphorylation site of Ser18. AA range:3-52
Specificity
Phospho-MRLC2 (S20) Polyclonal Antibody detects endogenous levels of MRLC2 protein only when phosphorylated at S20.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function
function:Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.,miscellaneous:This chain binds calcium.,PTM:Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains.,tissue specificity:Smooth muscle tissues and in some, but not all, nonmuscle cells.,
Protein name
Myosin regulatory light polypeptide 9
Abbreviation
Myosin Light Chain 2
Other name
MYL9; MLC2; MRLC1; MYRL2; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2; smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRL
Fields
>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Vascular smooth muscle contraction;>>Axon guidance;>>Focal adhesion;>>Tight junction;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Oxytocin signaling pathway;>>Shigellosis;>>Salmonella infection
Human gene ID
10398/10627
Human protein sequence Database
P24844
Mouse protein sequence database
Q9CQ19
Rat protein sequence database
Q64122
Cellular localization
Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Colocalizes with F-actin, MYH9 and PIEZO1 at the actomyosin cortex in myoblasts. .
Tissue expression
Smooth muscle tissues and in some, but not all, nonmuscle cells.
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