Immunogen
Synthetic Peptide of Flag-Tag
Specificity
The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.
Source
Mouse VH IgG1 and VL Kappa was expressed from 293F cells
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Dilution rate
WB: 1:10000-50000 ELISA 1:20000-50000 IP:100-1:200 IF: 1:1000 -3000
Purification process (Immunogen)
Protein A purified
Background
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Gene Name
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
Fields
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
Human protein sequence Database
Mouse protein sequence database
Rat protein sequence database
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