Molecular weight (DA)
66kD
Immunogen
Synthesized peptide derived from human Estrogen Receptor α. AA range:450-595
Specificity
This antibody detects endogenous levels of ESR1
Source
Monoclonal, Rabbit IgG1, Kappa
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Dilution rate
IHC 1:100-500, WB 1:500-1000, ELISA 1:5000-20000
Purification process (Immunogen)
Recombinant Expression and Affinity purified
Background
estrogen receptor 1(ESR1) Homo sapiens This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014],
Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter
Gene Name
Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1)
Protein name
Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1)
Other name
ESR1 ESR NR3A1
Human protein sequence Database
P03372
Mouse protein sequence database
P19785
Rat protein sequence database
P06211
Cellular localization
[Isoform 1]: Nucleus . Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . A minor fraction is associated with the inner membrane.; [Isoform 3]: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Single-pass type I membrane protein. Associated with the inner membrane via palmitoylation (Probable). At least a subset exists as a transmembrane protein with a N-terminal extracellular domain. .; Nucleus. Golgi apparatus. Cell membrane. Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated.
Tissue expression
Widely expressed (PubMed:10970861). Not expressed in the pituitary gland (PubMed:10970861). ; [Isoform 3]: Widely expressed, however not expressed in the pituitary gland.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
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