Molecular weight (DA)
15,30kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Caspase 6 around the phosphorylation site of Ser257. AA range:223-272
Specificity
Phospho-Caspase-6 (S257) Polyclonal Antibody detects endogenous levels of Caspase-6 protein only when phosphorylated at S257.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015],
Function
catalytic activity:Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-.,enzyme regulation:Activation is suppressed by phosphorylation at Ser-257.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.,PTM:Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit.,
Other name
CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2
Fields
>>Apoptosis;>>Lipid and atherosclerosis
Human protein sequence Database
P55212
Mouse protein sequence database
O08738
Rat protein sequence database
O35397
Cellular localization
Cytoplasm . Nucleus .
Tissue expression
Lung,Lymphocyte,T-cell,
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