Molecular weight (DA)
94kD
Immunogen
The antiserum was produced against synthesized peptide derived from human EXO1. AA range:61-110
Specificity
Exo1 Polyclonal Antibody detects endogenous levels of Exo1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008],
Function
cofactor:Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.,developmental stage:Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.,function:5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for
Protein name
Exonuclease 1
Other name
EXO1; EXOI; HEX1; Exonuclease 1; hExo1; Exonuclease I; hExoI
Human protein sequence Database
Q9UQ84
Mouse protein sequence database
Q9QZ11
Rat protein sequence database
Cellular localization
Nucleus . Colocalizes with PCNA to discrete nuclear foci in S-phase.
Tissue expression
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
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