Immunogen
Purified recombinant fragment of human BMAL1 expressed in E. Coli.
Specificity
BMAL1 Monoclonal Antibody detects endogenous levels of BMAL1 protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/200 - 1/1000. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
Affinity purification
Background
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],
Function
alternative products:Additional isoforms seem to exist,function:ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.,miscellaneous:CLOCK-ARNTL double mutations within the PAS domains result in syngernistic desensitization to high levels of CRY on repression of CLOCK-ARNTL transcriptional activity of PER1 and, disrupt circadian rhythmicity.,PTM:Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression.,PTM:Phosphorylated upon dimerization with CLOCK.,PTM:Sumoylated on Lys-259 upon dimerization with CLOCK.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 PAC (PAS-associated C-terminal) domain.,similarity:Contains 2 PAS (PER-ARNT-SIM) domains.,subunit:Component
Protein name
Aryl hydrocarbon receptor nuclear translocator-like protein 1
Other name
ARNTL; BHLHE5; BMAL1; MOP3; PASD3; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic-helix-loop-helix-PAS protein MOP3; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5; bHLHe5; Member of PAS protein
Fields
>>Circadian rhythm;>>Dopaminergic synapse
Human protein sequence Database
O00327
Mouse protein sequence database
Q9WTL8
Rat protein sequence database
Cellular localization
Nucleus . Cytoplasm . Nucleus, PML body . Shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer. The sumoylated form localizes in the PML body. Sequestered to the cytoplasm in the presence of ID2. .
Tissue expression
Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.
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