Molecular weight (DA)
30kD
Immunogen
Synthetic Peptide of CLIC1 AA range: 166-216
Specificity
CLIC1 protein(A216) detects endogenous levels of CLIC1
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC 1:100-200. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Background
chloride intracellular channel 1(CLIC1) Homo sapiens Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008],
Function
domain:Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.,function:Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions.,miscellaneous:The protein seems to have very low affinity for glutathion, even though glutathion binding was observed in protein crystals.,PTM:Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.,similarity:Belongs to the chloride channel CLIC family.,similarity:Contains 1 GST C-terminal domain.,su
Protein name
Chloride intracellular channel protein 1 (Chloride channel ABP) (Nuclear chloride ion channel 27) (NCC27) (Regulatory nuclear chloride ion channel protein) (hRNCC)
Other name
Chloride intracellular channel protein 1 (Chloride channel ABP;Nuclear chloride ion channel 27;NCC27;Regulatory nuclear chloride ion channel protein;hRNCC)
Human protein sequence Database
O00299
Mouse protein sequence database
Q9Z1Q5
Rat protein sequence database
Q6MG61
Cellular localization
Nucleus . Nucleus membrane ; Single-pass membrane protein . Cytoplasm . Cell membrane ; Single-pass membrane protein . Mostly in the nucleus including in the nuclear membrane (PubMed:9139710, PubMed:12681486). Small amount in the cytoplasm and the plasma membrane (PubMed:9139710). Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (PubMed:11940526, PubMed:11551966, PubMed:14613939). .
Tissue expression
Expression is prominent in heart, placenta, liver, kidney and pancreas.
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