Molecular weight (DA)
55kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MEF2D around the phosphorylation site of Ser444. AA range:410-459
Specificity
Phospho-MEF-2D (S444) Polyclonal Antibody detects endogenous levels of MEF-2D protein only when phosphorylated at S444.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012],
Function
developmental stage:Present in myotubes and also in undifferentiated myoblasts.,domain:The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.,function:Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.,PTM:Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.,PTM:Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity.
Protein name
Myocyte-specific enhancer factor 2D
Other name
MEF2D; Myocyte-specific enhancer factor 2D
Fields
>>cGMP-PKG signaling pathway;>>Apelin signaling pathway;>>Parathyroid hormone synthesis, secretion and action
Human protein sequence Database
Q14814
Mouse protein sequence database
Q63943
Rat protein sequence database
O89038
Cellular localization
Nucleus . Translocated by HDAC4 to nuclear dots.
Tissue expression
Blood,Brain,Epithelium,Eye,Myocardium,Testis,
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