Molecular weight (DA)
38kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Myogenic Factor 5 around the phosphorylation site of Ser49. AA range:21-70
Specificity
Phospho-Myf-5 (S49) Polyclonal Antibody detects endogenous levels of Myf-5 protein only when phosphorylated at S49.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
Function
function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
Protein name
Myogenic factor 5
Other name
MYF5; BHLHC2; Myogenic factor 5; Myf-5; Class C basic helix-loop-helix protein 2; bHLHc2
Fields
>>Signaling pathways regulating pluripotency of stem cells
Human protein sequence Database
P13349
Mouse protein sequence database
P24699
Rat protein sequence database
Cellular localization
Nucleus .
Tissue expression
Skeletal muscle,