Immunogen
The antiserum was produced against synthesized peptide derived from human DDX3Y. AA range:41-90
Specificity
DDX3Y Polyclonal Antibody detects endogenous levels of DDX3Y protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014],
Function
disease:DDX3Y is deleted in severe non-obstructive hypospermatogenesis [MIM:400042].,function:Probable ATP-dependent RNA helicase. May play a role in spermatogenesis.,similarity:Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,subcellular location:Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.,subunit:May interact with TDRD3.,tissue specificity:Testis-specific. Expressed predominantly in spermatogonia.,
Protein name
ATP-dependent RNA helicase DDX3Y
Other name
DDX3Y; DBY; ATP-dependent RNA helicase DDX3Y; DEAD box protein 3; Y-chromosomal
Human protein sequence Database
O15523
Mouse protein sequence database
Q62095
Rat protein sequence database
Cellular localization
Cytoplasm . Nucleus . Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.
Tissue expression
Widely expressed at the mRNA level, with highest levels in testis (PubMed:9381176). Testis-specific (at protein level). Expressed predominantly in spermatogonia, but occasionally detected in some pre-leptotene/leptotene spermatocytes (PubMed:15294876).
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