Immunogen
The antiserum was produced against synthesized peptide derived from human POLE1. AA range:1261-1310
Specificity
DNA pol ε A Polyclonal Antibody detects endogenous levels of DNA pol ε A protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
DNA polymerase epsilon, catalytic subunit(POLE) Homo sapiens This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013],
Function
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,domain:The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.,function:Participates in DNA repair and in chromosomal DNA replication.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the DNA polymerase type-B family.,subunit:Consists of two subunits (258 kDa and 55 kDa). Interacts with RAD17 and TOPBP1.,
Protein name
DNA polymerase epsilon catalytic subunit A
Other name
POLE; POLE1; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A
Fields
>>DNA replication;>>Base excision repair;>>Nucleotide excision repair
Human protein sequence Database
Q07864
Mouse protein sequence database
Q9WVF7
Rat protein sequence database
Cellular localization
Nucleus.
Tissue expression
Aorta endothelial cell,Colon,Kidney,Lung,T-cell,Testis,
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