Molecular weight (DA)
25kD
Immunogen
The antiserum was produced against synthesized peptide derived from human RPAB1. AA range:21-70
Specificity
POLR2E Polyclonal Antibody detects endogenous levels of POLR2E protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015],
Function
function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.,PTM:The N-terminus is blocked.,similarity:Belongs to the archaeal rpoH/eukaryotic RPB5 RNA polymerase subunit family.,subunit:Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase II
Protein name
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Other name
POLR2E; DNA-directed RNA polymerases I; II, and III subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4
Fields
>>RNA polymerase;>>Cytosolic DNA-sensing pathway;>>Huntington disease
Human protein sequence Database
P19388
Mouse protein sequence database
Q80UW8
Rat protein sequence database
B0BNE2
Cellular localization
Nucleus .
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