Molecular weight (DA)
127kD
Immunogen
The antiserum was produced against synthesized peptide derived from human SMG7. AA range:521-570
Specificity
SMG7 Polyclonal Antibody detects endogenous levels of SMG7 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
SMG7, nonsense mediated mRNA decay factor(SMG7) Homo sapiens This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011],
Function
function:Plays a role in nonsense-mediated mRNA decay. Recruits RENT1 to cytoplasmic mRNA decay bodies.,similarity:Contains 2 TPR repeats.,subcellular location:Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm.,subunit:Part of a complex that contains SMG5, SMG7, PP2AC, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated RENT1.,
Protein name
Protein SMG7
Other name
SMG7; C1orf16; EST1C; KIAA0250; Protein SMG7; EST1-like protein C; SMG-7 homolog; hSMG-7
Fields
>>mRNA surveillance pathway
Human protein sequence Database
Q92540
Mouse protein sequence database
Q5RJH6
Rat protein sequence database
Cellular localization
Cytoplasm . Nucleus . Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm. .
Tissue expression
Bone marrow,Brain,Epithelium,Testis,
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