Molecular weight (DA)
42kD
Immunogen
The antiserum was produced against synthesized peptide derived from human TF2A1. AA range:281-330
Specificity
TFIIA-α Polyclonal Antibody detects endogenous levels of TFIIA-α protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010],
Function
function:TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.,PTM:The alpha and beta subunits are postranslationally produced from the precursor form by TASP1. The cleavage promotes proteosomal degradation.,similarity:Belongs to the TFIIA subunit 1 family.,subunit:TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12). TFIIA forms a complex with TBP.,
Protein name
Transcription initiation factor IIA subunit 1
Other name
GTF2A1; TF2A1; Transcription initiation factor IIA subunit 1; General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42
Fields
>>Basal transcription factors;>>Viral carcinogenesis
Human protein sequence Database
P52655
Mouse protein sequence database
Q99PM3
Rat protein sequence database
O08949
Cellular localization
Nucleus.
Tissue expression
Epithelium,
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车