Immunogen
Purified recombinant human Aldose Reductase protein fragments expressed in E.coli.
Specificity
Aldose Reductase Monoclonal Antibody detects endogenous levels of Aldose Reductase protein.
Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009],
Function
catalytic activity:Alditol + NAD(P)(+) = aldose + NAD(P)H.,disease:In diabetes and galactosemia, increased AR activity leads to high levels of sorbitol and galactitol, respectively, in the cells of many tissues. Accumulation of sugar alcohols has been shown to cause osmotic cataracts in the lens. AR is also thought to play a key role in diabetic complications of three other target tissues, namely, nerve, kidney and retina.,enzyme regulation:Cys-299 may regulate the kinetic and inhibition properties of the enzyme, but does not participate in catalysis.,function:Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.,similarity:Belongs to the aldo/keto reductase family.,subunit:Monomer.,tissue specificity:Highly expressed in embryonic epithelial cells (EUE) in response to osmoti
Protein name
Aldose reductase
Other name
AKR1B1; ALDR1; Aldose reductase; AR; Aldehyde reductase; Aldo-keto reductase family 1 member B1
Fields
>>Pentose and glucuronate interconversions;>>Fructose and mannose metabolism;>>Galactose metabolism;>>Glycerolipid metabolism;>>Folate biosynthesis;>>Metabolic pathways
Human protein sequence Database
P15121
Mouse protein sequence database
P45376
Rat protein sequence database
P07943
Cellular localization
Cytoplasm.
Tissue expression
Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
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