Immunogen
Purified recombinant human ATP-citrate synthase (C-terminus) protein fragments expressed in E.coli.
Specificity
ATP-citrate synthase Monoclonal Antibody detects endogenous levels of ATP-citrate synthase protein.
Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Immunofluorescence: 1/100 - 1/500. Flow cytometry: 1/100 - 1/200. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014],
Function
catalytic activity:ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.,function:ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.,similarity:In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.,similarity:In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.,subunit:Homotetramer.,
Protein name
ATP-citrate synthase
Abbreviation
ATP-citrate synthase
Other name
ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme
Fields
>>Citrate cycle (TCA cycle);>>Metabolic pathways
Human protein sequence Database
P53396
Mouse protein sequence database
Q91V92
Rat protein sequence database
P16638
Cellular localization
Cytoplasm, cytosol .
Tissue expression
Brain,Epithelium,Hippocampus,Liver,Lymph,Platelet,
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