Immunogen
Purified recombinant human CYP7A1 (C-terminus) protein fragments expressed in E.coli.
Specificity
CYP7A1 Monoclonal Antibody detects endogenous levels of CYP7A1 protein.
Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010],
Function
catalytic activity:Cholesterol + NADPH + O(2) = 7-alpha-hydroxycholesterol + NADP(+) + H(2)O.,cofactor:Heme group.,online information:Cholesterol-7 alpha-hydroxylase entry,pathway:Lipid metabolism; bile acid biosynthesis.,similarity:Belongs to the cytochrome P450 family.,
Protein name
Cholesterol 7-alpha-monooxygenase
Other name
CYP7A1; CYP7; Cholesterol 7-alpha-monooxygenase; CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1
Fields
>>Primary bile acid biosynthesis;>>Steroid hormone biosynthesis;>>Metabolic pathways;>>PPAR signaling pathway;>>Bile secretion;>>Cholesterol metabolism
Human protein sequence Database
P22680
Mouse protein sequence database
Q64505
Rat protein sequence database
Cellular localization
Endoplasmic reticulum membrane ; Single-pass membrane protein . Microsome membrane ; Single-pass membrane protein .
Tissue expression
Detected in liver.
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