Immunogen
The antiserum was produced against synthesized peptide derived from human p40 phox around the phosphorylation site of Thr154. AA range:120-169
Specificity
Phospho-p40-phox (T154) Polyclonal Antibody detects endogenous levels of p40-phox protein only when phosphorylated at T154.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000 ,IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding d
Function
function:Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 1 SH3 domain.,subunit:p40-PHOX associates primarily with p67-PHOX to form a complex with p47-PHOX.,tissue specificity:Expression is restricted to hematopoietic cells.,
Protein name
Neutrophil cytosol factor 4
Other name
NCF4; SH3PXD4; Neutrophil cytosol factor 4; NCF-4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox; p40phox
Fields
>>Phagosome;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Prion disease;>>Leishmaniasis;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis
Human protein sequence Database
Q15080
Mouse protein sequence database
P97369
Rat protein sequence database
Cellular localization
Cytoplasm, cytosol . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Membrane ; Peripheral membrane protein .
Tissue expression
Expression is restricted to hematopoietic cells.
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