Molecular weight (DA)
46kD
Immunogen
The antiserum was produced against synthesized peptide derived from human ACOT1. AA range:91-140
Specificity
ACOT1 Polyclonal Antibody detects endogenous levels of ACOT1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer.,
Function
catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer.,
Protein name
Acyl-coenzyme A thioesterase 1
Other name
ACOT1; CTE1; Acyl-coenzyme A thioesterase 1; Acyl-CoA thioesterase 1; CTE-I; CTE-Ib; Inducible cytosolic acyl-coenzyme A thioester hydrolase; Long chain acyl-CoA thioester hydrolase; Long chain acyl-CoA hydrolase
Fields
>>Fatty acid elongation;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Ovarian steroidogenesis
Human protein sequence Database
Q86TX2
Mouse protein sequence database
O55137
Rat protein sequence database
Cellular localization
Cytoplasm, cytosol .
Tissue expression
Neuroblastoma,
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