Molecular weight (DA)
48kD
Immunogen
The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410
Specificity
ACOT4 Polyclonal Antibody detects endogenous levels of ACOT4 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.,similarity:Belongs to the C/M/P thioester hydrolase family.,
Function
catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.,similarity:Belongs to the C/M/P thioester hydrolase family.,
Protein name
Acyl-coenzyme A thioesterase 4
Other name
ACOT4; PTE2B; PTEIB; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib
Fields
>>Fatty acid elongation;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Ovarian steroidogenesis
Human protein sequence Database
Q8N9L9
Mouse protein sequence database
Q8BWN8
Rat protein sequence database
Cellular localization
Peroxisome .
Tissue expression
Strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle.
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