Molecular weight (DA)
85kD
Immunogen
The antiserum was produced against synthesized peptide derived from human IREB1 around the phosphorylation site of Ser711. AA range:681-730
Specificity
Phospho-IRP-1 (S711) Polyclonal Antibody detects endogenous levels of IRP-1 protein only when phosphorylated at S711.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct
Function
catalytic activity:Citrate = isocitrate.,cofactor:Binds 1 4Fe-4S cluster per subunit.,function:Binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. This protein also expresses aconitase activity.,online information:Aconitase entry,similarity:Belongs to the aconitase/IPM isomerase family.,
Protein name
Cytoplasmic aconitate hydratase
Other name
ACO1; IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
Fields
>>Citrate cycle (TCA cycle);>>Glyoxylate and dicarboxylate metabolism;>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids
Human protein sequence Database
P21399
Mouse protein sequence database
P28271
Rat protein sequence database
Q63270
Cellular localization
Cytoplasm, cytosol .
Tissue expression
Brain,Brain astrocytoma,Uterus,
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车