Molecular weight (DA)
40kD
Immunogen
The antiserum was produced against synthesized peptide derived from human DNAJB11. AA range:31-80
Specificity
ERdj3 Polyclonal Antibody detects endogenous levels of ERdj3 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a soluble glycoprotein of the endoplasmic reticulum (ER) lumen that functions as a co-chaperone of binding immunoglobulin protein, a 70 kilodalton heat shock protein chaperone required for the proper folding and assembly of proteins in the ER. The encoded protein contains a highly conserved J domain of about 70 amino acids with a characteristic His-Pro-Asp (HPD) motif and may regulate the activity of binding immunoglobulin protein by stimulating ATPase activity. [provided by RefSeq, Mar 2014],
Function
caution:PubMed:11584023 reported a cytosolic, as well as nuclear subcellular location. This result was obtained using an N-terminally GFP-tagged construct which most probably affected signal peptide-driven targeting to the ER. As a consequence, the in vivo revelance of the observed interaction with APOBEC1, a nuclear protein, is dubious. This holds true for the interaction with PWP1.,function:Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.,induction:By ER stress-inducing agents, such as thapsigargin and tunicamycin.,PTM:Contains high-mannose Endo H-sensitive carbohydrates.,PTM:Cys-169, Cys-171, Cys-193 and Cys-196
Protein name
DnaJ homolog subfamily B member 11
Other name
DNAJB11; EDJ; ERJ3; HDJ9; PSEC0121; DnaJ homolog subfamily B member 11; APOBEC1-binding protein 2; ABBP-2; DnaJ protein homolog 9; ER-associated DNAJ; ER-associated Hsp40 co-chaperone; ER-associated dnaJ protein 3; ERdj3; ERj3p; HEDJ; Human
Fields
>>Protein processing in endoplasmic reticulum
Human protein sequence Database
Q9UBS4
Mouse protein sequence database
Q99KV1
Rat protein sequence database
Q6TUG0
Cellular localization
Endoplasmic reticulum lumen . Associated with the ER membrane in a C-terminally epitope-tagged construct.
Tissue expression
Widely expressed.
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