Molecular weight (DA)
42kD
Immunogen
The antiserum was produced against synthesized peptide derived from human FUT3. AA range:91-140
Specificity
FucT-III Polyclonal Antibody detects endogenous levels of FucT-III protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008],
Function
catalytic activity:GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->4))-N-acetyl-beta-D-glucosaminyl-R.,function:May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme.,miscellaneous:Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.,online information:Blood group antigen gene mutation database,online information:Fucosyltransferase 3,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 10 family.,subcellular location:Membrane
Protein name
Galactoside 3(4)-L-fucosyltransferase
Other name
FUT3; FT3B; LE; Galactoside 3(4)-L-fucosyltransferase; Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III; FucT-III
Fields
>>Glycosphingolipid biosynthesis - lacto and neolacto series;>>Metabolic pathways
Human protein sequence Database
P21217
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein . Membrane-bound form in trans cisternae of Golgi.
Tissue expression
Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.
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