Molecular weight (DA)
31kD
Immunogen
The antiserum was produced against synthesized peptide derived from human GCSm-gamma. AA range:42-91
Specificity
GCSm-γ Polyclonal Antibody detects endogenous levels of GCSm-γ protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015],
Function
pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain.,tissue specificity:In all tissues examined. Highest levels in skeletal muscle.,
Protein name
Glutamate--cysteine ligase regulatory subunit
Other name
GCLM; GLCLR; Glutamate--cysteine ligase regulatory subunit; GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit
Fields
>>Cysteine and methionine metabolism;>>Glutathione metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors;>>Ferroptosis
Human protein sequence Database
P48507
Mouse protein sequence database
O09172
Rat protein sequence database
P48508
Cellular localization
cytosol,glutamate-cysteine ligase complex,
Tissue expression
In all tissues examined. Highest levels in skeletal muscle.
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