Molecular weight (DA)
30kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPL47. AA range:191-240
Specificity
MRP-L47 Polyclonal Antibody detects endogenous levels of MRP-L47 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identifie
Function
sequence caution:Chimeric cDNA. Seems to include a portion of the orthologous murine sequence.,similarity:Belongs to the ribosomal protein L47em family.,
Protein name
39S ribosomal protein L47 mitochondrial
Other name
MRPL47; NCM1; CGI-204; 39S ribosomal protein L47; mitochondrial; L47mt; MRP-L47; Nasopharyngeal carcinoma metastasis-related protein 1
Human protein sequence Database
Q9HD33
Mouse protein sequence database
Q8K2Y7
Rat protein sequence database
Cellular localization
Mitochondrion .
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