Molecular weight (DA)
20kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS25. AA range:124-173
Specificity
MRP-S25 Polyclonal Antibody detects endogenous levels of MRP-S25 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],
Function
similarity:Belongs to the ribosomal protein S25/L51 family.,subunit:Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.,
Protein name
28S ribosomal protein S25 mitochondrial
Other name
MRPS25; RPMS25; 28S ribosomal protein S25; mitochondrial; MRP-S25; S25mt
Human protein sequence Database
P82663
Mouse protein sequence database
Q9D125
Rat protein sequence database
Q4QR80
Cellular localization
Mitochondrion .
Tissue expression
Lung,Spleen,
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