Immunogen
Synthesized Acetyl peptide derived from human ACSS1. at AA range: K642
Specificity
This antibody detects endogenous levels of ACSS1 at Human:K642;Mouse:K635;Rat:K635, It doesn't reacte with total protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],
Function
catalytic activity:ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.,function:Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).,sequence caution:Sequencing errors.,similarity:Belongs to the ATP-dependent AMP-binding enzyme family.,
Gene Name
ACSS1 ACAS2L KIAA1846
Other name
Acetyl-coenzyme A synthetase 2-like, mitochondrial (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) (AceCS2) (Acyl-CoA synthetase short-chain family member 1)
Fields
>>Glycolysis / Gluconeogenesis;>>Pyruvate metabolism;>>Glyoxylate and dicarboxylate metabolism;>>Propanoate metabolism;>>Metabolic pathways;>>Carbon metabolism
Human protein sequence Database
Q9NUB1
Mouse protein sequence database
Q99NB1
Rat protein sequence database
Cellular localization
Mitochondrion matrix .
Tissue expression
Amygdala,Brain,PCR rescued clones,Placenta,Spleen,Stomach,T
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