Immunogen
Recombinant Protein of RFP-Tag
Specificity
The antibody detects RFP ERFP tag fusion proteins. It isn't reactive against GFP CFP YFP. It is reactive against morange tag weakly.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Dilution rate
WB: 1:10000-20000,IF 1:200-2000
Purification process (Immunogen)
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
RFP (Red Fluorescent Protein), cloned from Discosoma coral (DsRed, DsRed2 or drFP583), is a protein which exhibits bright red fluorescence when exposed to blue light. It is common research practice for biologists to introduce a gene (or a gene chimera) encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy.
Human protein sequence Database
Mouse protein sequence database
Rat protein sequence database