Molecular weight (DA)
37kD
Immunogen
Synthesized peptide derived from Yeast GAPDH
Specificity
This antibody detects endogenous levels of Yeast GAPDH
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.66% sodium azide.
Dilution rate
IHC-p1:50-200 ,WB 1:1000-2000
Purification process (Immunogen)
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Human protein sequence Database
P00360
Mouse protein sequence database
Rat protein sequence database