Molecular weight (DA)
70kD
Immunogen
Synthesized peptide derived from human protein . at AA range: 190-270
Specificity
PABP4 Polyclonal Antibody detects endogenous levels of protein.
Formulation
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Dilution rate
WB 1:500-2000 ELISA 1:5000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript va
Function
function:Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo.,PTM:Arg-518 is dimethylated, probably to asymmetric dimethylarginine.,similarity:Belongs to the polyadenylate-binding protein type-1 family.,similarity:Contains 1 PABC domain.,similarity:Contains 4 RRM (RNA recognition motif) domains.,subunit:Interacts with NFX1.,tissue specificity:Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.,
Gene Name
PABPC4 APP1 PABP4
Protein name
Polyadenylate-binding protein 4 (PABP-4) (Poly(A)-binding protein 4) (Activated-platelet protein 1) (APP-1) (Inducible poly(A)-binding protein) (iPABP)
Fields
>>mRNA surveillance pathway;>>RNA degradation
Human protein sequence Database
Q13310
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Tissue expression
Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.
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