Molecular weight (DA)
42kD
Immunogen
Synthesized peptide derived from human SPHK1 (Phospho-Ser225)
Specificity
This antibody detects endogenous levels of SPHK1 (Phospho-Ser225) at Human, Mouse,Rat
Source
Polyclonal, Rabbit,IgG
Formulation
Liquid in PBS containing 50% glycerol, and 0.110% sodium azide.
Dilution rate
WB 1:500-2000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Function
catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.,similarity:Contains 1 DAGKc domain.,subunit:Interacts with ACY1 (By similarity). Binds to calmodulin. Interacts with SPHKAP.,tissue specificity:Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.,
Protein name
SPHK1 (Phospho-Ser225)
Other name
Sphingosine kinase 1 (SK 1) (SPK 1) (EC 2.7.1.91)
Fields
>>Sphingolipid metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Tuberculosis
Human protein sequence Database
Q9NYA1
Mouse protein sequence database
Q8CI15
Rat protein sequence database
Q91V26
Cellular localization
Cytoplasm . Nucleus . Cell membrane . Endosome membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse . Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner (PubMed:19854831). Binds to membranes containing negatively charged lipids but not neutral lipids (PubMed:24929359). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity). .
Tissue expression
Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level) (PubMed:29662056).
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