Molecular weight (DA)
113kD
Immunogen
Synthesized peptide derived from human PARP-1. AA range: 410-460
Specificity
This antibody detects endogenous levels of human PARP-1
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC-p 1:50-200, WB 1:500-2000. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],
Function
catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT
Gene Name
PARP1 ADPRT PPOL
Other name
Poly [ADP-ribose] polymerase 1 (PARP-1;EC 2.4.2.30;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD(+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1)
Fields
>>Base excision repair;>>NF-kappa B signaling pathway;>>Apoptosis;>>Necroptosis;>>Diabetic cardiomyopathy
Human protein sequence Database
P09874
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. .
Tissue expression
Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,
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