Immunogen
Synthesized peptide derived from human PUS10 AA range: 425-475
Specificity
This antibody detects endogenous levels of PUS10 at Human/Mouse
Source
Polyclonal, Rabbit,IgG
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009],
Function
catalytic activity:tRNA uridine = tRNA pseudouridine.,function:Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Partially unspliced pre-RNA.,similarity:Belongs to the pseudouridine synthase Pus10 family.,
Gene Name
PUS10 CCDC139 DOBI
Human protein sequence Database
Q3MIT2
Mouse protein sequence database
Q9D3U0
Rat protein sequence database
Cellular localization
Nucleus . Cytoplasm . Mitochondrion . Localizes mainly in the nucleus (Probable) (PubMed:31819270). tRNA pseudouridylate synthase activity is restricted to the cytoplasm (PubMed:31819270). Translocates from nucleus to mitochondria during TRAIL-induced apoptosis (PubMed:28981101). .
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车