Immunogen
Synthesized peptide derived from human SIA4A AA range: 189-239
Specificity
This antibody detects endogenous levels of SIA4A at Human/Mouse
Source
Polyclonal, Rabbit,IgG
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq, Jul 2008],
Function
catalytic activity:CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R.,function:It may be responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found on sugar chains O-linked to Thr or Ser and also as a terminal sequence on certain gangliosides. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.,online information:GlycoGene database,online information:ST3Gal I,pathway:Protein modification; protein glycosylation.,PTM:The soluble form derives from the membrane form by proteolytic processing.,similarity:Belongs to the glycosyltransferase 29 family.,subcellular location:Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.,tissue specificity:Expressed in several tissues. Highest
Gene Name
ST3GAL1 SIAT4 SIAT4A
Fields
>>Mucin type O-glycan biosynthesis;>>Glycosaminoglycan biosynthesis - keratan sulfate;>>Glycosphingolipid biosynthesis - globo and isoglobo series;>>Glycosphingolipid biosynthesis - ganglio series;>>Metabolic pathways
Human protein sequence Database
Q11201
Mouse protein sequence database
P54751
Rat protein sequence database
Cellular localization
Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein . Golgi apparatus, trans-Golgi network membrane ; Single-pass type II membrane protein . Secreted. Membrane-bound form in medial and trans cisternae of Golgi (PubMed:9182658). Secreted into the body fluid. .
Tissue expression
Expressed in several tissues. Highest expression in lung, liver, skeletal muscle, kidney, pancreas, spleen and placenta.
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