Immunogen
Purified recombinant fragment of human KLHL12 expressed in E. Coli.
Specificity
KLHL12 Monoclonal Antibody detects endogenous levels of KLHL12 protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],
Function
domain:The BTB domain is required for interaction with CUL3.,function:Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subunit:Component of an ubiquitin-protein E3 ligase complex which includes at least CUL3 and KLHL12. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A.,tissue specificity:Highly expressed in testis and at lower levels in the submandibular salivary gland.,
Protein name
Kelch-like protein 12
Other name
KLHL12; C3IP1; Kelch-like protein 12; CUL3-interacting protein 1; DKIR homolog; hDKIR
Human protein sequence Database
Q53G59
Mouse protein sequence database
Q8BZM0
Rat protein sequence database
Cellular localization
Cytoplasmic vesicle, COPII-coated vesicle .
Tissue expression
Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland.
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