Molecular weight (DA)
85kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MAK around the phosphorylation site of Tyr159. AA range:126-175
Specificity
Phospho-MAK (Y159) Polyclonal Antibody detects endogenous levels of MAK protein only when phosphorylated at Y159.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Could play an important function in spermatogenesis.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed mainly in testicular cells at and after meiosis.,
Protein name
Serine/threonine-protein kinase MAK
Other name
MAK; Serine/threonine-protein kinase MAK; Male germ cell-associated kinase
Human protein sequence Database
P20794
Mouse protein sequence database
Q04859
Rat protein sequence database
P20793
Cellular localization
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cell projection, cilium, photoreceptor outer segment . Photoreceptor inner segment. Localized in both the connecting cilia and the outer segment axonemes (By similarity). Localized uniformly in nuclei during interphase, to the mitotic spindle and centrosomes during metaphase and anaphase, and also to midbody at anaphase until telophase. .
Tissue expression
Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
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