Immunogen
The antiserum was produced against synthesized peptide derived from human DGKH. AA range:771-820
Specificity
DGK-η Polyclonal Antibody detects endogenous levels of DGK-η protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
diacylglycerol kinase eta(DGKH) Homo sapiens This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014],
Function
catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,function:Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).,PTM:Phosphorylated; does not inhibit catalytic activity.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subcellular location:Translocated from the cytoplasm to endosomes in response to stress stimuli. Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association.,subunit:Isoform 1 forms homooligomers through the SAM domain. Isoform 1 is also able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization of isoform
Protein name
Diacylglycerol kinase eta
Other name
DGKH; Diacylglycerol kinase eta; DAG kinase eta; Diglyceride kinase eta; DGK-eta
Fields
>>Glycerolipid metabolism;>>Glycerophospholipid metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Phospholipase D signaling pathway;>>Choline metabolism in cancer
Human protein sequence Database
Q86XP1
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Cytoplasm . Cell membrane . Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association (PubMed:12810723). Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS (PubMed:19710016).
Tissue expression
[Isoform 1]: Expressed only in testis, kidney and colon. ; [Isoform 2]: Ubiquitously expressed.
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