Molecular weight (DA)
70kD
Immunogen
The antiserum was produced against synthesized peptide derived from human PLK3. AA range:231-280
Specificity
Fnk Polyclonal Antibody detects endogenous levels of Fnk protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.,induction:Cytokine and cellular adhesion trigger FNK induction.,PTM:Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 POLO box domains.,subunit:Binds to the calcium/integrin-binding protein (CIB). This interaction probably occurs via the POLO-box domain.,tissue specificity:Transcripts are highly detected in placenta, lung, followed by skeletal mu
Protein name
Serine/threonine-protein kinase PLK3
Other name
PLK3; CNK; FNK; PRK; Serine/threonine-protein kinase PLK3; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3; Proliferation-related kinase
Fields
>>FoxO signaling pathway;>>C-type lectin receptor signaling pathway;>>Tuberculosis
Human protein sequence Database
Q9H4B4
Mouse protein sequence database
Q60806
Rat protein sequence database
Q9R011
Cellular localization
Cytoplasm. Nucleus. Nucleus, nucleolus. Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase. According to a report, PLK3 localizes only in the nucleolus and not in the centrosome, or in any other location in the cytoplasm (PubMed:17264206). The discrepancies in results may be explained by the PLK3 antibody specificity, by cell line-specific expression or post-translational modifications. .
Tissue expression
Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
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