Molecular weight (DA)
100kD
Immunogen
The antiserum was produced against synthesized peptide derived from human PIK3R5. AA range:695-744
Specificity
PI 3-kinase p101 Polyclonal Antibody detects endogenous levels of PI 3-kinase p101 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011],
Function
domain:The heterodimerization region allows the binding to the catalytic subunit.,enzyme regulation:Greatly activated by G gamma proteins.,function:Regulatory subunit of the PI3K gamma complex.,subunit:Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. Interacts with G beta gamma proteins.,tissue specificity:Highly expressed in leukocytes, followed by spleen, lymph node, thymus ans bone marrow.,
Protein name
Phosphoinositide 3-kinase regulatory subunit 5
Abbreviation
PI 3-kinase p101
Other name
PIK3R5; Phosphoinositide 3-kinase regulatory subunit 5; PI3-kinase regulatory subunit 5; PI3-kinase p101 subunit; Phosphatidylinositol 4; 5-bisphosphate 3-kinase regulatory subunit; PtdIns-3-kinase regulatory subunit; Protein FOAP-2; PtdIns-
Fields
>>cGMP-PKG signaling pathway;>>Chemokine signaling pathway;>>Phospholipase D signaling pathway;>>PI3K-Akt signaling pathway;>>Adrenergic signaling in cardiomyocytes;>>Apelin signaling pathway;>>Platelet activation;>>Cholinergic synapse;>>Oxytocin signaling pathway;>>Toxoplasmosis;>>Kaposi sarcoma-associated herpesvirus infection
Human protein sequence Database
Q8WYR1
Mouse protein sequence database
Q5SW28
Rat protein sequence database
Cellular localization
Nucleus . Cytoplasm . Cell membrane ; Peripheral membrane protein . Predominantly localized in the nucleus in absence of PIK3CG/p120. Colocalizes with PIK3CG/p120 in the cytoplasm. Translocated to the plasma membrane in a beta-gamma G protein-dependent manner. .
Tissue expression
Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli.
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