Immunogen
Synthesized peptide derived from human Neuron-Specific Enolase(NSE)
Specificity
This antibody detects endogenous levels of human Neuron-Specific Enolase(NSE). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH9.0 was highly recommended as antigen repair method in paraffin sect
Source
Mouse, Monoclonal/IgG1, Kappa
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC-p 1:100-500, WB 1:200-1000
Purification process (Immunogen)
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
enolase 2(ENO2) Homo sapiens This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008],
Function
catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.,cofactor:Magnesium. Required for catalysis and for stabilizing the dimer.,developmental stage:During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.,function:Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.,induction:Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jacob disease.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.,similarity:Belongs to the enolase family.,subcellular location:Can translocate to the plasma membrane
Protein name
Neuron-Specific Enolase(NSE)
Other name
Gamma-enolase (EC 4.2.1.11;2-phospho-D-glycerate hydro-lyase;Enolase 2;Neural enolase;Neuron-specific enolase;NSE)
Fields
>>Glycolysis / Gluconeogenesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of amino acids;>>RNA degradation;>>HIF-1 signaling pathway
Human protein sequence Database
P09104
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Cytoplasm . Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. .
Tissue expression
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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