Immunogen
The antiserum was produced against synthesized peptide derived from human ARHGEF11. AA range:198-247
Specificity
PDZ-RhoGEF Polyclonal Antibody detects endogenous levels of PDZ-RhoGEF protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
Function
domain:The poly-Pro region is essential for plasma membrane localization upon stimulation.,function:May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane upon stimulation.,subunit:Interacts with GNA12 and GNA13 through the RGS domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts with SLC1A6.,tissue specificity:Ubiquitously expressed.,
Protein name
Rho guanine nucleotide exchange factor 11
Other name
ARHGEF11; KIAA0380; Rho guanine nucleotide exchange factor 11; PDZ-RhoGEF
Fields
>>Vascular smooth muscle contraction;>>Parathyroid hormone synthesis, secretion and action;>>Pathogenic Escherichia coli infection;>>Human cytomegalovirus infection;>>Pathways in cancer
Human protein sequence Database
O15085
Mouse protein sequence database
Rat protein sequence database
Q9ES67
Cellular localization
Cytoplasm . Membrane . Translocated to the membrane upon stimulation.
Tissue expression
Ubiquitously expressed.
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