Molecular weight (DA)
73kD
Immunogen
Synthesized peptide derived from Rap1GAP . at AA range: 460-540
Specificity
Rap1GAP Polyclonal Antibody detects endogenous levels of Rap1GAP protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
RAP1 GTPase activating protein(RAP1GAP) Homo sapiens This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011],
Function
function:GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.,induction:By 12-O-tetradecanoylphorbol-13-acetate (TPA) in promyelocytic HL-60 cells.,similarity:Contains 1 GoLoco domain.,similarity:Contains 1 Rap-GAP domain.,tissue specificity:Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.,
Protein name
Rap1 GTPase-activating protein 1
Other name
RAP1GAP; KIAA0474; RAP1GA1; Rap1 GTPase-activating protein 1; Rap1GAP; Rap1GAP1
Fields
>>Rap1 signaling pathway
Human protein sequence Database
P47736
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Golgi apparatus membrane; Peripheral membrane protein.
Tissue expression
Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
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