Molecular weight (DA)
77kD
Immunogen
The antiserum was produced against synthesized peptide derived from human DBF4. AA range:10-59
Specificity
Dbf4 Polyclonal Antibody detects endogenous levels of Dbf4 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
function:Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.,induction:Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 DBF4-type zinc finger.,similarity:Contains 2 BRCT domains.,subunit:Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2L, ORC4L and ORC6L.,tissue specificity:Highly expressed in testis and thymus. Expressed also in most cancer cells lines.,
Function
function:Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.,induction:Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 DBF4-type zinc finger.,similarity:Contains 2 BRCT domains.,subunit:Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2L, ORC4L and ORC6L.,tissue specificity:Highly expressed in testis and thymus. Expressed also in most ca
Protein name
Protein DBF4 homolog A
Other name
DBF4; ASK; DBF4A; ZDBF1; Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1
Human protein sequence Database
Q9UBU7
Mouse protein sequence database
Q9QZ41
Rat protein sequence database
Cellular localization
Nucleus .
Tissue expression
Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
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