Molecular weight (DA)
55kD
Immunogen
The antiserum was produced against synthesized peptide derived from human NIPA. AA range:320-369
Specificity
NIPA Polyclonal Antibody detects endogenous levels of NIPA protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis. [provided by RefSeq, Aug 2013],
Function
caution:Reported to contain a F-box domain (PubMed:16009132). Such domain is however not predicted by any detection method.,developmental stage:Weakly expressed in G0/G1 phases, abundant during S and G2/M phases, and strongly decreases thereafter.,domain:The F-box-like region is required for the interaction with SKP1A.,function:Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated.
Protein name
Nuclear-interacting partner of ALK
Other name
ZC3HC1; NIPA; HSPC216; Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1
Human protein sequence Database
Q86WB0
Mouse protein sequence database
Q80YV2
Rat protein sequence database
Cellular localization
Nucleus .
Tissue expression
Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.
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