Molecular weight (DA)
136kD
Immunogen
The antiserum was produced against synthesized peptide derived from human NID1. AA range:219-268
Specificity
Nidogen Polyclonal Antibody detects endogenous levels of Nidogen protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
nidogen 1(NID1) Homo sapiens This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008],
Function
function:Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.,PTM:N- and O-glycosylated.,similarity:Contains 1 NIDO domain.,similarity:Contains 1 nidogen G2 beta-barrel domain.,similarity:Contains 1 thyroglobulin type-1 domain.,similarity:Contains 4 LDL-receptor class B repeats.,similarity:Contains 6 EGF-like domains.,subunit:Interacts with FBLN1 and LGALS3BP.,
Other name
NID1; NID; Nidogen-1; NID-1; Entactin
Human protein sequence Database
P14543
Mouse protein sequence database
P10493
Rat protein sequence database
P08460
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane.
Tissue expression
Brain,Placenta,Testis,
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