Molecular weight (DA)
52kD
Immunogen
Synthesized peptide derived from human NSUN6
Specificity
This antibody detects endogenous levels of NSUN6 at Human, Mouse
Source
Polyclonal, Rabbit,IgG
Formulation
Liquid in PBS containing 50% glycerol, and 0.600% sodium azide.
Dilution rate
WB 1:500-2000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) . In vitro also methylates tRNA(Thr)(AGT) . Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates .
Protein name
Putative methyltransferase NSUN6 (EC 2.1.1.-) (NOL1P2/Sun and PUA domain-containing protein 1) (NOL1P2/Sun domain family member 6)
Human protein sequence Database
Q8TEA1
Mouse protein sequence database
Q7TS68
Rat protein sequence database
Cellular localization
Cytoplasm .
Storage
‘-15°C to -25°C/1 year(Do not lower than -25°C)