Molecular weight (DA)
44kD
Immunogen
Synthesized peptide derived from human SPO11
Specificity
This antibody detects endogenous levels of SPO11 at Human, Mouse
Source
Polyclonal, Rabbit,IgG
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Function
Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2.
Protein name
Meiotic recombination protein SPO11 (Cancer/testis antigen 35) (CT35)
Human protein sequence Database
Q9Y5K1
Mouse protein sequence database
Q9WTK8
Rat protein sequence database
Cellular localization
Nucleus .
Tissue expression
Highly expressed in testis.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)